New study highlights the antioxidant activity of sulfated glucosamine

The following study reveals one potential mechanism through which sulfated glucosamine may act to suppress osteoarthritis. This particular study highlights the antioxidant activity of sulfated glucosamine on reducing oxidative stress in chondrocytes (cartilage cells). The implications of this study include the possibility that glucosamine may actually reduce celluar damage caused by oxidative strees on chondrocytes and therefore potentially act not only as a symptom modifying agent, but more importantly as a disease modifying agent in the treatment of osteoarthritis.

"Although, several effects of glucosamine and its sulfated form (sulfated glucosamine) have been proposed for the suppression of osteoarthritis, their exact mechanisms have not been completely elucidated. This study explains the novel possibility of involvement of sulfated glucosamine in improving cellular antioxidant potential and thereby controlling oxidative damage that could be effective for its therapeutic potential in osteoarthritis.

Treatment with sulfated glucosamine to human chondrocytes and macrophages inhibited radical simulated oxidation of membrane lipids, proteins and DNA in a dose-dependent manner. Moreover, detection of reactive oxygen species by electron spin resonance (ESR) spectroscopy and 2',7'-dichlorodihydrofluororescein diacetate (DCFH-DA) fluorescence probe clearly confirmed effective radical scavenging potential of sulfated glucosamine in cellular and non-cellular systems.

More importantly, NF-kappaB reporter gene assay and western blot analysis revealed that sulfated glucosamine inhibits radical mediated expression and activation of nuclear factor kappaB (NF-kappaB) proteins (transcription factor involves in expression of a number of genes related to osteoarthritis). Further, sulfated glucosamine enhanced reduced glutathione (GSH) level in oxidatively stressed human chondrocytes improving cellular redox balance. In conclusion, it is suggested that potential effects of sulfated glucosamine in controlling osteoarthritis might be partly via mechanisms involving direct scavenging of cellular radical species and alteration of oxidation mediated destructive events" (Menis et al, 2007).

Reference:

Mendis E, Kim MM, Rajapakse N, Kim SK. Sulfated glucosamine inhibits oxidation of biomolecules in cells via a mechanism involving intracellular free radical scavenging. Eur J Pharmacol. 2007 Oct 25;